Immunohistochemistry is based on the technique that interaction between the antigen and the antibody. This technique is used to identify the antigens present in the given tissue sample by using the antigen- antibody specificity in binding. This technique was initially designed by Dr. Albert Coons in 1941. This is technique is widely used in the diagnosis of abnormal cell growth, like cancerous tissues. In these tissue types the biding is based upon the specific binding of biomarkers that are expressed by the cell during particular events of the cell like apoptosis. Based upon this differential protein expression the binding is found. Once the particular antigen is given the binding can be visualized by number of ways like an enzyme conjugate assay can be performed where enzymes like peroxidase can be used to determine the reaction or a flurophore can be used. Hence visualization of these reactions helps the doctors to identify the causative agant and hence proceed towards appropriate treatment.
Last date updated on July, 2014