Peer review refers to the work done during the screening of submitted manuscripts and funding applications. This process encourages authors to meet the accepted standards of their discipline and reduces the dissemination of irrelevant findings, unwarranted claims, unacceptable interpretations, and personal views. Publications that have not undergone peer review are likely to be regarded with suspicion by academic scholars and professionals.
Cell culture is the complex process by which the cells are grown under controlled conditions. A consistent presence of floating cells is a common phenomenon in cultures of human embryonic stem cells (hESCs). It is currently believed that unavoidable imperfections in culture conditions lead the cells to undergo senescence and apoptosis resulting in unattached cells floating in the culture medium. Inspired by recent studies on mitotic activities in human embryonic stem cell colonies, we believe the existence of floating cells is not simply the result of unfavorable growth conditions but an intrinsic phenomenon resulted from maintaining the pluripotency of hESCs under the culture conditions. Continuous cell division across the colonies is responsible for the emergence of floating cells during hESC culture. This is quite different from the bacterial colony growth where the cells in the center of colonies are quiescent. Results indicates that continuous cell division, even at the costof floating cells formation, is essential for human embryonic stem cell proliferation.(Chen L, Jin Q, Gong Jand Krishna Dasa SS, The Nature of Floating Cells in Human Embryonic Stem Cell Culture)
Last date updated on December, 2020