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conferenceseries.com
Volume 5, Issue 5 (Suppl) Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5th International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
LC-MS-based metabolomics study on the different growth stages of Hermetia illucens L.
Chen-Wei Su and Ching-Kuo Lee
Taipei Medical University, Taiwan
Hermetia Illucens L. (HI), the species of Stratiomyidae insects, which lives by livestock manure and kitchen waste. Due to
abundance of protein they can be used to produce high-value animal protein feed. In addition, there are many advantages,
i.e., reproduce rapidly, euryphagous except human food, raise easily and low cost. Based on the above, HI is a good media for
resource transformation. In this study, we applied LC-MS technique combined with statistical analysis (PCA, OPLS-DA) to explore
the different growth stages of HI. The growth stages of the larvae are five; the freshly hatched larvae were sacrificed and freeze-dried
as the blank of zero stage. The rest of larvae were reared and fed on Sesamum Indium (SI) and they would enter the next stage by each
peeling. We took the second, fourth and fifth stages by each peeling a week for analysis. According to the LC-MS data, we run the
database software-Compound discoverer 2.0 to get predicted compounds information like retention time, chemical formula and area
under the curve. After obtaining the information, we had run statistical software-SIMCAP to analyze the difference among SI and
HI, HI with fed SI. In summary, the results from LC-MS technique combined with statistical analysis can be speculated that the use
of energy insects Hermetia illucens L. is not only amino acids but also free fatty acids.
Biography
Chen-Wei Su had completed his Bachelor’s degree from Pharmacy and Science of Chia Nan University and joined Taipei Medical University for Master’s degree.
Chen-Wei Su et al., Nat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017