"For the decolorization experiments, five agar plugs (6 mm diameter) of active mycelium from PDA plate was transferred aseptically into 500 ml Erlenmeyer flasks containing 100 ml of autoclaved PDB medium and RBBR (60mg L -1 ) or the dye effluent at different concentration of 30% (v/v). The pH of PDB medium was adjusted using 1.0 M NaOH or 1.0 M HCl. The cultures were incubated at 28Â°C in rotary shakers running at 150 rpm. Biotic (sterilized medium, without dye addition) and abiotic (sterilized medium containing the effluent but not inoculated with the fungus) control experimental sets were also prepared and maintained in parallel with the decolorization experiments.
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Last date updated on July, 2014