Epitope mapping is the process of identification of the molecular determinants for antibody-antigen recognition on the antigen. Identification of the epitope is a key step in the characterization of monoclonal antibodies, especially those used in therapeutic strategies. It has also been essential in the design of vaccines against toxins or against enzymes. To obtain information on sequential and/or assembled epitopes, various approaches have been developed in conjunction with mass spectrometry. Fine epitope mapping could be achieved by this approach with microgram quantities of antibodies and in a short time compared to conventional mapping methods.
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Last date updated on July, 2014