"A large-scale closed system culture within which cells square measure full-grown in an exceedingly fastened volume of nutrient substance below specific environmental conditions (e.g. nutrient sort, temperature, pressure, aeration, etc.) up to a particular density in an exceedingly tank or airlift fermenter, harvested and processed as a batch, particularly before all nutrients square measure dried-up. In batch cultivation, the bacterium square measure inoculated into the bioreactor (always stirred tank bioreactor). Then, below bound conditions (temperature, pH, aeration, etc.) the bacterium undergo all the expansion phases (lag, exponential, stationary). At last, the fermentation is stopped and the merchandise is collected. Then, once improvement and sterilization of the fermenter, the fermenter is prepared for one more batch.
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Last date updated on January, 2021