"A method for monitoring a biotechnological fermentation using cyclic voltammetry in combination with chemometrics, commonly termed electronic tongue, is proposed. A simple palladized palladium electrode showed excellent sensitivity to glucose concentrations ranging from 0 to 9 g/L in a yeast fermentation medium (pH 5.3,0.07 M chloride). This electrode is also sensitive to ethanol, but not able to separate it from the glucose signal. To separately quantify mixtures of glucose and ethanol as the product of yeast fermentations at least one additional electrode with different sensitivities is needed. Platinized platinum shows the required features. Long term stability is still an obstacle for application. Analysis of biotechnological fermentations is a hot topic in such diverse industries as pharma, food, bio-fuel production and wastewater treatment to name a few. A common problem for both running established fermentations as well as testing new ones is monitoring of the process parameters, as even slight aberrations can lead to reduced yield or even a complete failure. Therefore it is highly desirable to have a powerful real-time online monitoring system available to allow for timely intervention when a process parameter starts to run off limits.
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Last date updated on December, 2020