|Development of active logarithmic microbial culture that is suitable for the final industrial production level is known as inoculum development. Inoculum we use for industrial fermentations should be in its active, healthy and exponential growth phase, it should be available free of contamination in required large volumes, it should retain its capability of formation of desired product formation. The process is a stepwise and gradual increase in scaling-up the volume of inoculum to the desired level, which includes preparation of bacterial suspension (either vegetative cells or spores) in sterile tap water and then to the broth or in case of fungi, their hyphae are transferred to the broth. This is usually done using flask cultures ranging in between 50 ml to 12 lt and volume of the flask or container can be increased as per the need. Volume of inoculum we add to fermenter should be about 5% to that of media volume. Major pitfalls we should be aware of are the risk of contamination and the degeneration of strain.
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