|Methodology applied for product recovery depends upon when and where the product is formed, the purity of the product, use of the product, impurities that are present within the broth, price of the product and so on. âWhenâ is applicable for, with which phase of the cell cycle the product is formed and âwhereâ is applicable for, whether the product is intra-cellular or extra-cellular in its formation. Product recovery cost mainly guides the cost of the product in the market. More investment towards product recovery increases the cost of the product in the market. Even purity of the product and intended use of the product will increase the investment towards product recovery and purification. Various techniques involved in product recovery are filtration, centrifugation, precipitation, dialysis, solvent extraction, chromatographic and electrophoretic techniques, crystallization techniques etc. Products which is extra-cellular, need filtration of spent broth directly and if the product is intra-cellular, then the cells need to be lysed either by sonication or by centrifugation methods and then the resultant product is subjected to filtration and other purification techniques.
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