The efficacy of the restriction fragment length polymorphism analysis of polymerase chain reaction amplified ribosomal ribonucleic acid for the identification of Bacillus spp. isolated from freshwater pond ecosystems was evaluated. Results showed that no restriction site differences occur between rrs operons within the bacteria belonging to the same species and as a result members of the same species yield very similar RFLP patterns at 100% similarity level. Cluster analysis of PCR-RFLP patterns grouped all the isolates into three clusters; each cluster represented a single species except one for which no placement was obtained. Phenotypic characteristics of the isolates were found to be well concordance with the results of PCR-RFLP. Our findings suggested that PCR- RFLP is suitable tool for discrimination and identification of endoglucanase positive Bacillus spp. isolated from freshwater ecosystems.
The impact factor of journal provides quantitative assessment tool for grading, evaluating, sorting and comparing journals of similar kind. It reflects the average number of citations to recent articles published in science and social science journals in a particular year or period, and is frequently used as a proxy for the relative importance of a journal within its field. It is first devised by Eugene Garfield, the founder of the Institute for Scientific Information. The impact factor of a journal is evaluated by dividing the number of current year citations to the source items published in that journal during the previous two years.
Last date updated on July, 2014