"F. oxysporum was maintained on solid PDA medium and after 7 days the spores were isolated by pouring sterile distilled water (5-6 ml) to the plate and scrapping it gently with sterile loop to release the spores. The resulting spore suspension was filtered through two layers of sterile cheese cloth to remove any mycelial fragments and the conidial suspension was centrifuged at 4,500 rpm, 4ÂºC for 5 min.The spores were washed thrice with sterile water to remove the spore mucilage and finally resuspended in sterile water at a concentration of Ã106 spores/ml. The spores were germinated for 6 h in PDA broth at 28ÂºC at 100 rpm.
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Last date updated on July, 2014