Fungal Spore Collection

"F. oxysporum was maintained on solid PDA medium and after 7 days the spores were isolated by pouring sterile distilled water (5-6 ml) to the plate and scrapping it gently with sterile loop to release the spores. The resulting spore suspension was filtered through two layers of sterile cheese cloth to remove any mycelial fragments and the conidial suspension was centrifuged at 4,500 rpm, 4ºC for 5 min.The spores were washed thrice with sterile water to remove the spore mucilage and finally resuspended in sterile water at a concentration of ×106 spores/ml. The spores were germinated for 6 h in PDA broth at 28ºC at 100 rpm. OMICS Group International is one of the leading Open Access Publishers which is publishing 700+ peer-reviewed journals with the support of 50,000+ editorial board members as editorial team and aimed to disseminate the scholarly knowledge to the scientific society. OMICS Group also organizing 3000+ International Scientific Conferences and events yearly all over the world with the support of 1000+ Scientific associations worldwide.

• Share this page
• 
• 
• 
• 
• 
• 

Last date updated on July, 2025