"Implementing a particular transformation strategy depends on the fungal strain and needs to be optimized to achieve the significant transformation efficiency. Although AMT method is the commonly available method for transformation of F. oxysporum, here we report an alternative novel transformation system for this fungus. This method uses glass beads to deliver DNA molecules in combination with polyethylene glycol. It is a simple, rapid and cost-effective method that worked efficiently in F. oxysporum. In this study, we utilized there porter gene, GFP under the control of cauliflower mosaic virus35S constitutive promoter for optimizing the transformation procedure. In addition, we also transformed the fungus with GFP gene by AMT method, which enabled us to compare the two methods of transformation. Although, the transformation efficiency achieved with AMT and glass bead transformation methods were high, the glass bead method yielded relatively higher transformation frequency with higher fluorescence intensity in most of the fungal transformed isolates as compared to AMT method.
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Last date updated on July, 2014