"Genomic DNA was isolated from the transfromants and PCR analysis was done to study the presence of transgenes using GFP and HPT gene specific primers, and the expected amplicon size of 500 bp was obtained. The GFP expression in the transformants was checked by semi-quantitative RT-PCR using GFP primers. Some of the transformants obtained by AMT method showed high GFP expression like Fo.AT3, 4 and 5 as compared to other transformants in which the expression ranged from low to very negligible.The transformants obtained by glass bead method were also analyzed for GFP expression and transformants Fo.G5, 6, 7 showed high GFP expressions, while Fo.G3 and 4 were low expressing transformants and some like Fo.G1, 2 and 8 did not exhibit any expression. The variation in GFP expression in different transformed isolates may bedue to the position effect of the transgene integration and dosage effect of the transgene copy number.
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Last date updated on July, 2014