alexa Glass Bead Transformation|Journals Open Access|glass Bead Transformation

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Glass Bead Transformation

"The important factor considered while applying glass bead method for fungal transformation was the size of the glass beads. The glass beads with 0.45-0.52 mm diameter were found to be suitable for transformation. The ratio of number of spores to the amount of glass beads is also crucial, and the ratio of 1:1 yielded better transformation efficiency. Prior to transformation, the plasmid DNA was linearized with EcoRI restriction enzyme. Polyethylene glycol (PEG 3500) was added to the transformation mixture at a final concentration of 5% wt/ vol and 0.1 M lithium chloride was also added to facilitate the DNA uptake. The use of PEG results into clumping of treated cells which facilitate the trapping of DNA [7], whereas the use of high concentration of lithium ions renders the cell walls permeable to DNA without the need for protoplasts, a method initially applied for transforming yeast, S. cerevisiae. OMICS Group International is one of the leading Open Access Publishers which is publishing 700+ peer-reviewed journals with the support of 50,000+ editorial board members as editorial team and aimed to disseminate the scholarly knowledge to the scientific society. OMICS Group also organizing 3000+ International Scientific Conferences and events yearly all over the world with the support of 1000+ Scientific associations worldwide.
 
  • Share this page
  • Facebook
  • Twitter
  • LinkedIn
  • Google+
  • Pinterest
  • Blogger
Last date updated on July, 2014