"The same sample as population-based sequencing was used for high-throughput sequencing (HTS). HIV-1 RNA was extracted from 1 ml of plasma using QIA amp viral RNA extraction kits (Qiagen, Courtaboeuf, France). Reverse-transcription was performed with random primers and Superscript III RT (Invitrogen, Carlsbad, CA) followed by nested PCR, the PCR product was then purified (QIAquick Gel Extraction Kit ).
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Last date updated on July, 2014