Hippocampal Neurons

Hippocampal neurons were obtained from embryonic day 18 (E-18) rats and prepared as previously described. Briefly, hippocampi were dissected from the fetuses of timed-pregnant Sprague-Dawley rats. The tissue was mechanically dissociated in a cold serum free dissection solution that contained Hibernate E medium supplemented with B27 (2% v/v; Invitrogen), glutamax (1% v/v; Invitrogen) and antibiotic/antimycotic (1% v/v; Invitrogen). Prior to cell plating each MEA was sterilized with absolute ethanol and dried. Cells were plated onto 10 MEAs divided into two groups. OMICS Group International is one of the leading Open Access Publishers which is publishing 700+ peer-reviewed journals with the support of 50,000+ editorial board members as editorial team and aimed to disseminate the scholarly knowledge to the scientific society.

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Last date updated on September, 2024