Hippocampal neurons were obtained from embryonic day 18 (E-18) rats and prepared as previously described. Briefly, hippocampi were dissected from the fetuses of timed-pregnant Sprague-Dawley rats. The tissue was mechanically dissociated in a cold serum free dissection solution that contained Hibernate E medium supplemented with B27 (2% v/v; Invitrogen), glutamax (1% v/v; Invitrogen) and antibiotic/antimycotic (1% v/v; Invitrogen). Prior to cell plating each MEA was sterilized with absolute ethanol and dried. Cells were plated onto 10 MEAs divided into two groups.
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Last date updated on July, 2014