"To mimic the ethanol-dehydration step typically performed during histological processing of FFPE tissues, the formaldehyde-fixed RNase A was precipitated nd incubated in 100% ethanol for 1 hr, 24 hr, or 1 week. SDS-PAGE revealed that the formaldehyde-fixed, ethanol-treated samples were as highly cross-linked as the original formaldehyde-fixed samples. However, after heating in the Tris-SDS recovery differ at 100Â°C for 20 min, followed by 60Â°C for 2 hr, no reversal of the formaldehyde-induced cross-links was observed.
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Citation: Fowler CB, OâLearyTJ, MasonJT (2014) Improving the ProteomicAnalysis of Archival Tissue by Using Pressure-Assisted Protein Extraction:A Mechanistic Approach.J Proteomics Bioinform7: 151-157."
Last date updated on July, 2014