Brain sections were incubated with dihydroethidium (DHE) (stock solution, 1 mM in dimethyl sulfoxide, diluted to 5 μm with PBS, Molecular Probes, Eugene, OR, USA) in a humidified chamber, at 37°C for 30 minutes. Coronal brain sections were cut into 40 μm. A set of brain sections was processed for immunohistochemistry with polyclonal rabbit antimyeloperoxidase antibody (1:200, Thermo Fisher Scientific, Fremont, CA, USA) at 4°C overnight. Body weight measurements and neurologic evaluations were assessed at 72 hours after the ischemiaâreperfusion insult by one observer unaware of treatment protocol.
Peer review refers to the work done during the screening of submitted manuscripts and funding applications. This process encourages authors to meet the accepted standards of their discipline and reduces the dissemination of irrelevant findings, unwarranted claims, unacceptable interpretations, and personal views. Publications that have not undergone peer review are likely to be regarded with suspicion by academic scholars and professionals.
Last date updated on September, 2014
