Cryopreservation is one of the most widespread preservation techniques used for the maintenance of microbial culture collections, and has been successfully used to store a number of obligate biotrophs . A key step in the methods reported involves the partial dehydration of spores, followed by storage at low temperature. Several specialized dehydration treatments have been developed for obligate biotrophic pathogens. For example, the conidia of Uncinula necator were left to air-dry for approximately 18 hours in a laminar flow cabinet before being snap frozen in liquid nitrogen, while the conidia of Podosphaera fusca were desiccated with CaCl2 before treatment with liquid nitrogen.
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Citation: Gong S, Yang L, Shi W, Xue M, Meng Q, et al. (2013) Cryopreservation of Silica Gel-dried Conidia of Blumeria graminis f. sp. Tritici. J Plant Pathol Microb 4: 206.
Last date updated on October, 2020