Three hundred Dolichos bean (Lablab purpureus) genotypes were screened against Dolichos Yellow Mosaic Virus (DYMV) disease. Initial screening was done under field conditions, where disease incidence was calculated for each genotype. Subsequently, selfed progenies of 34 symptomless lines were challenged by sap inoculation under field conditions, out of which only three genotypes, viz. VRSEM-894, VRSEM-887 and VRSEM-860 did not show any symptoms. Using root stalk of susceptible genotype (Ankur Goldy), these three putative symptomless genotypes were further challenged by grafting. The resistant reactions of VRSEM-894, VRSEM-887 and VRSEM-860 were confirmed, as even after 60 days of successful grafting, no viral symptom appeared on all the grafted plants of these genotypes. When subjected to PCR amplification with DYMV coat protein gene specific primer, these three symptomless genotypes did not show any amplification, suggesting that there was no infection of Dolichos Yellow Mosaic Virus in those genotypes.
OMICS Group International is one of the leading Open Access Publishers which is publishing 700+ peer-reviewed journals with the support of 50,000+ editorial board members as editorial team and aimed to disseminate the scholarly knowledge to the scientific society. OMICS Group also organizing 3000+ International Scientific Conferences and events yearly all over the world with the support of 1000+ Scientific associations worldwide.
Citation: Singh PK, Ashish Kumar, Rai N, Singh DV (2012) Identification of Host Plant Resistant to Dolichos Yellow Mosaic Virus (DYMV) in Dolichos Bean (Lablab Purpureus). J Plant Pathol Microb 3:130.
Last date updated on March, 2021