Elicitins are also sterol carrier proteins which bind sterols and catalyze their transfer between membranes. The initial complex of elicitin binding to sterols was confirmed by the crystal structures of cryptogein in complex with dehydroergosterol (DHE). Binding of elicitin to ligands appears to be essential for induction of a biological response in plants. Interaction between the protein and ergosterol involves several residues, among which tyrosine residues are the most denoted. In this paper, we report the amplification, cloning and complete gene information of elicitin gene from P. capsici infecting black pepper. In addition, we used the complete genome sequence information of P. capsici to identify the Transcription Start Site (TSS) and promoters. Further, the docking analysis revealed the binding mode of capsicein and ergosterol at atomic level.
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Citation: Vijesh Kumar IP, Reena N, Anandaraj M, Eapen SJ, Johnson GK, et al. (2013) Amplification, Cloning and In silico Prediction of Full Length Elicitin Gene from Phytophthora capsici, the Causal Agent of Foot Rot Disease of Black Pepper. J Plant Pathol Microb 4: 181
Last date updated on July, 2014