Sporangia were produced by transferring agar plugs (10Ã10 mm2) from actively growing area of 10-day-old cultures on CV8A to Petri dishes containing non-sterile, 1.5% soil water extract solution (SWE, 15g of sandy loam soil/1 L distilled water) or filtered, non-sterile pond water. Mature sporangia developed after incubating at room temperature (c. 23Â°C) under cool-white fluorescent light. Sporangia and gametangia were photographed with a Nikon Fujix Digital Camera HC-300Zi connected to a Nikon Labophot-2 microscope. Fifty randomly selected mature sporangia were measured for length and width while 30 gametangia were measured for the size of oogonia, oospores, and antheridia with Image-ProÂ® Plus v. 22.214.171.124.
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Citation: Yang X, Copes WE, Hong C (2013) Phytophthora mississippiae sp. nov., a New Species Recovered from Irrigation Reservoirs at a Plant Nursery in Mississippi. J Plant Pathol Microb 4: 180
Last date updated on July, 2014