Genetic diversity among 41 isolates was determined using vegetative compatibility groups from different regions of Fars (Firozabad, Ramjerd, Drodzan, Shiraz, Pasargad, Sepidan and Mravdasht) and Khuzestan (Ezh, Ramhormoz and Ahodasht) provinces. Nitrate non-utilizing (Nit) mutants were generated on Czapeck media, containing 5% KClO3. Phenotypic classes of nit mutants were determined to 17 different Vegetative Compatibility Groups (VCGs) on media containing nitrate, hypoxanthine and ammonium. The largest group contained 18 isolates and others contained two and one, respectively. 24 isolates were selected for RAPD test, to represent 17 vegetative compatibility groups. A set of seven random primers revealed a total of 36 alleles. High level of genetic diversity was observed among F. verticillioides isolated in the region. 29 alleles (80.55) showed high polymorphism in all the isolates. Genetic relatedness was calculated based on genetic distance matrix, and 11 groups were clustered based on UPGMA and Dice coefficient.
OMICS Group International is one of the leading Open Access Publishers which is publishing 700+ peer-reviewed journals with the support of 50,000+ editorial board members as editorial team and aimed to disseminate the scholarly knowledge to the scientific society. OMICS Group also organizing 3000+ International Scientific Conferences and events yearly all over the world with the support of 1000+ Scientific associations worldwide.
Citation: Bahmani Z, Nejad RF, Nourollahi K, Fayazi F, Mahinpo V (2012) Investigation of Fusarium verticillioides on the Basis of RAPD Analysis, and Vegetative Compatibility in Iran. J Plant Pathol Microb 3:147.
Last date updated on September, 2024
Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi