"Liquid chromatography-mass spectrometry (LC/MS)-based proteomics, efficient extraction of high-quality proteins is of keyimportance. Most current extraction protocols for FFPE proteomics have been adapted from heat-induced antigen retrieval methods, originally developed for immunohistochemistry. Highly encouraging proteomic studies of FFPE tissues have appeared in the recent literature and there are several comprehensive reviews ofthis topic. However these investigations have typically been restricted to minute tissue specimens, such as those obtained by laser capture micro-dissection. Further, some studies report high rates ofalse-positive protein identification and are limited to the analysis of tryptic digests of FFPE tissues by LC/MS. To develop improve dextraction protocols for FFPE tissue; our laboratory has taken a mechanistic approach, by first studying the reactions of formaldehyde.
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Citation: Fowler CB, OâLearyTJ, MasonJT (2014) Improving the ProteomicAnalysis of Archival Tissue by Using Pressure-Assisted Protein Extraction:A Mechanistic Approach.J Proteomics Bioinform7: 151-157."
Last date updated on May, 2021