ARMS, also named allele specific PCR, is a diagnostic technique for the detection of single base mutation including both germ-line and somatic mutations. It is simple and reliable for detection of single nucleotide polymorphisms (SNPs). ARMS is performed by using a pair of PCR primers that are specific to either the mutant or the wild type sequences. The polymorphic nucleotides should be present at the 3' end of the primers to enhance the specificity of the reaction. Extension would only occur if the 3' end of the allele specific primer is bound to the complementary target sequences. Polymerases with no 3â endonuclease activities are required to prevent correction of terminal mismatch. The genotype of SNPs is determined by analysis of PCR products with gel electrophoresis.
Journal of Medical Diagnostic Methods is one of the top online publishing journals for research from the field of Amplification Refractory Mutation System. The open access articles published in this scholarly journal are reviewed by at least two reviewers of the associated fields. The journal publishes original research articles, reviews, case reports, short communications, etc and emphasizes theoretical and experimental work. To ensure high quality articles, both editors and reviewers actively participate in the peer review process and help in completing the review process within 21 days. As an important educational platform the journal provides scientists and researchers access to the newest trends and research outlooks. Please browse through the list of peer-reviewed journals to find the scientific publication of your interest.
Last date updated on September, 2014