alexa Bactericidal Assay|OMICS Group|Medical Diagnostic Methods

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Bactericidal Assay

Phagocytosis and Microbicidal activity were evaluated by the acridine orange method described by Bellinati-Pires et al. Equal volumes of bacteria and cell suspensions were mixed and incubated at 37°C for 30 min under continuous shaking. Phagocytosis was stopped by incubation in ice. To eliminate extracellular bacteria, the suspensions were centrifuged twice (160 × g, 10 min, 4°C). Cells were resuspended in serum-free 199 medium and centrifuged. The supernatant was discarded and the sediment dyed with 200μL of acridine orange (Sigma, ST Loius, USA; 14.4g/L) for 1 min. The sediment was resuspended in cold culture 199 medium, washed twice and observed under immunofluorescence microscope at 400X and 1000X magnification. Journal of Medical Diagnostic Methods is one of the top online publishing journals for research from the field of Bactericidal Assay. The open access articles published in this scholarly journal are reviewed by at least two reviewers of the associated fields. The journal publishes original research articles, reviews, case reports, short communications, etc and emphasizes theoretical and experimental work. To ensure high quality articles, both editors and reviewers actively participate in the peer review process and help in completing the review process within 21 days. As an important educational platform the journal provides scientists and researchers access to the newest trends and research outlooks. Please browse through the list of peer-reviewed journals to find the scientific publication of your interest.
 
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