alexa High-Resolution-Melting-Analysis|OMICS International|Medical Diagnostic Methods

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High-Resolution-Melting-Analysis

High resolution melting (HRM) is a powerful post-PCR technique that reveals and distinguishes point mutations according to the difference in melting temperature (Tm) and is subsequently confirmed using sequencing. HRM makes use of intercalating dyes such as LCGreen, which emit light when intercalated into double-stranded DNA (ds DNA) but only fluoresce at low level in the absence of dsDNA. Unlike SYBR green, LCGreen does not inhibit amplification. The melting analysis is first introduced with the Light-Cycler and is reported to be performed on other HRM instruments. Journal of Medical Diagnostic Methods is one of the top online publishing journals for research from the field of High Resolution Melting Analysis. The open access articles published in this scholarly journal are reviewed by at least two reviewers of the associated fields. The journal publishes original research articles, reviews, case reports, short communications, etc and emphasizes theoretical and experimental work. To ensure high quality articles, both editors and reviewers actively participate in the peer review process and help in completing the review process within 21 days. As an important educational platform the journal provides scientists and researchers access to the newest trends and research outlooks. Please browse through the list of peer-reviewed journals to find the scientific publication of your interest.
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Last date updated on September, 2014

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