Within the past centuries laboratory diagnostic has rapidly evolved in a variety of fields, for instance in clinical chemistry. In comparison to clinical chemistry, microbiological diagnostic is relatively slow because time consuming bacterial growth is essential. Bacterial growth is necessary to obtain populations consisting of only one bacterial species, e.g., when samples are examined physiologically containing various species of microorganisms. Moreover, species identification commonly relies on the ability of microorganisms to metabolize energy sources, e.g., various sugars, and also these analyses are time consuming. Within the past years MALDI-TOF mass spectrometry, previously used especially in research laboratories, has been developed right up to usage in commercial high throughput labs allowing acceleration of species identification even in routine diagnostics. Reich et al. describe implementation of this new technique in a commercial lab for microbiological analyses.
Diagnostic Methods of Microbial Pathogens- Stefan Borgmann
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Last date updated on June, 2014