alexa Microorganisms|Journals Open Access|Microorganisms

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"Phosphate-solubilizing microorganisms are isolated from rhizospheric samples by plating serial dilutions of rhizospheric soil extracts in NBRIP solid medium (glucose 10g, MgCl2.6H2O 5g, MgSO4.7H2O 0.25g, KCl 0.2 g and (NH4)2 SO4 0.1g, agar 15g, H2O 1L) supplemented with 5g/l of TCP. That medium contains insoluble tri-calcium phosphate allowing the detection of phosphate solubilizer microorganisms by the formation of “halos” around their colonies. All plates were incubated at 30°C for 1 week. Colonies surrounded by solubilized clear zone were picked and streaked onto NBRIP plates containing 5g/l of TCP. Plates were again incubated at the same conditions to confirm their abilities to solubilize insoluble phosphate. Stock cultures were routinely maintained on LB agar supplemented with 1% of glucose. OMICS Group International is one of the leading Open Access Publishers which is publishing 350 scholarly open access journals with the support of 30,000 well-versed scholars as editorial team and aimed to disseminate the scholarly knowledge to the scientific society. OMICS Group also organizing 100 international scientific conferences and events yearly all over the world with the support of 100+ scientific associations worldwide.
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Last date updated on July, 2014