|"Phosphate-solubilizing microorganisms are isolated from rhizospheric samples by plating serial dilutions of rhizospheric soil extracts in NBRIP solid medium (glucose 10g, MgCl2.6H2O 5g, MgSO4.7H2O 0.25g, KCl 0.2 g and (NH4)2 SO4 0.1g, agar 15g, H2O 1L) supplemented with 5g/l of TCP. That medium contains insoluble tri-calcium phosphate allowing the detection of phosphate solubilizer microorganisms by the formation of âhalosâ around their colonies. All plates were incubated at 30Â°C for 1 week. Colonies surrounded by solubilized clear zone were picked and streaked onto NBRIP plates containing 5g/l of TCP. Plates were again incubated at the same conditions to confirm their abilities to solubilize insoluble phosphate. Stock cultures were routinely maintained on LB agar supplemented with 1% of glucose.
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