Extra pulmonary tuberculosis (TB) comprises 15% of the total tuberculosis cases. In cases of suspected extrapulmonary tuberculosis, rapid and accurate laboratory diagnosis is of prime importance, since traditional techniques of detecting acid-fast bacilli have limitations. The major difficulty with mycobacteria in tissue samples is achieving optimal cell lysis. A comparison of two methods, pretreatment of tissue with 4% Sodium Hypochlorite in Bleach concentration method and pretreatment with petroffs method before culture on Lowenstein Jensen medium, was conducted on 18 extrapulmonary tissue specimens collected from different sites of suspected TB patients to evaluate the use of Bleach concentration method in tissue samples. The aim is to apply this method for demonstration of AFB in tissue samples obtained from extrapulmonary sites and to correlate with Ziehl Neelson staining and LJ culture. All the samples were processed for conventional ZN staining, bleach concentration method and AFB culture on LJ media.
Last date updated on June, 2014