The cellular metabolic activity seemed affected by the AuNPs, thus, the possibility of apoptosis induction by the nanoparticles was assessed. DNA fragmentation was performed for the tumour cells treated with purified gold nanoparticle and it was determined by visualization of DNA laddering on agarose gel electrophoresis.Biofunctionalizedbioconjugate gold nanoparticles treatment on HepG2 and A549 cells were stained with Annexin-V and morphological changes were immediately observed under fluorescence microscopy. HepG2 cell treated with 82.91nM and A549 cell treated with 144.16nM of gold nanoparticles, showed an increase of propidium iodide positive dead cell.and cells which are both propidium iodide and Annexin VFITC positive (scatted greens orange); this indicates that treatment of gold nanoparticle in HepG2 cells ,positive A549 cells and treated A549 cancer cells caused apoptosis. The staining confirmed the efficiency of biofunctionalized gold nanoparticles causing apoptosis. (Manoj Singh, Manish Kumar, Manikandan S, Chandrasekaran N, Amitava Mukherjee and AK Kumaraguru, Drug Delivery System for Controlled Cancer Therapy Using Physico-Chemically Stabilized Bioconjugated Gold Nanoparticles Synthesized from Marine Macroalgae, PadinaGymnospora)
Impact Factor is an indicator of the number of citations within the scientific journals of clinical and medical category. Impact factor is measured based on the frequency with which a journal's articles are cited in scientific publications, is a putative marker of journal quality. Publication ccitation, index of researchers, and journal impact factors are used to measure the impact & importance of research. Journal Impact factor are most widely used metric for measuring the quality of clinical Journal.
Clinical Journals impact factor can be improved by using various study methods, includes assigning more referees to check the quality of the submitted articles and thus nominating their journals for indexing in databases.
Last date updated on July, 2014