"Using buccal swaps, preserved dried blood spots, hair, urine, fecal or amniotic samples for DNA isolation often requires whole genome amplification steps to obtain the desired amounts of genomic DNA fragments. Buccal cells have been collected for genetic studies using cytology brushes, mouthwash or treated cards. Cytobrushes provided the best DNA in terms of quality and quantity. While these methods as well as cheek scraping with tongue depressors work well for PCR based experiments, the DNA quality from buccal cells is poor and not suitable for DNA genotyping. A significant disadvantage is the need for whole genome amplification for most genotyping projects. In addition, buccal cell samples are thought not to perform accurately for CNV detection because of the abundance of bacterial DNA.
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Last date updated on July, 2014