alexa A Comprehensive Method of Identifying Heat Shock Proteins (HSPs)
e-ISSN:2320-1215 p-ISSN: 2322-0112

Research & Reviews in Pharmacy and Pharmaceutical Sciences
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Research Article

A Comprehensive Method of Identifying Heat Shock Proteins (HSPs)

Pielesz A1*, Machnicka A2, Binias D1 and Sarna E1

University of Bielsko-Biala, Faculty of Materials, Civil and Environmental Engineering, Bielsko-Biala, Poland

Department of Microbiology and Environmental Technology, Institute of Engineering and Environmental Protection, Faculty of Materials, Civil and Environmental Engineering, University of Bielsko-Biala, Poland

*Corresponding Author:
Pielesz A
University of Bielsko-Biala, Faculty of Materials, Civil and Environmental Engineering, Bielsko-Biala, Poland, Tel: 48 33 82–27114 E-mail: apielesz@ath.bielsko.pl

Received: 02/02/2016 Accepted: 29/02/2016 Published: 06/03/2016

 

 

Abstract

Because no model will ever completely replicate clinical human wound healing, it is essential that the model utilized be selected with care. Anatomically and physiologically, poultry skin is similar to human skin in many respects. Therefore, organic chicken skin (an ex-vivo burninjured skin model) was analysed in this study. Acetate electrophoresis (CAE), microbiological procedure, Fourier-transform infrared spectrometry (FTIR) and scanning electron microscopy analysis (SEM) were all carried out after heating samples of model chicken skin to a temperature simulating a burn incident and stimulating the release of Heat Shock Proteins (HSPs). Aggregates of smaller molecular weight, HSP37 proteins, were isolated by cellulose acetate electrophoresis. FTIR tests revealed that heating a dry organic chicken skin to boiling point leads to the production of β-sheet aggregates, which are the response of protein to thermal shock. Aggregates of HSP37 are produced in thermal injury and not all the antimicrobial activity of the skin is lost in this model. So, antimicrobial peptides found in the burnt skin, HSP proteins were confirmed by microscopic, microbiological, electrophoretic and spectroscopic examination.

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