alexa A Rapid Sensitive Detection Method by Plate Assay for C
E- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286

Research & Reviews: Journal of Microbiology and Biotechnology
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Research Article

A Rapid Sensitive Detection Method by Plate Assay for Catalase Activity from Bacterium Acinetobacter calcoaceticus AV6

Vijayakumar Arul Doss1, Murugesan Subban2*, Jayapal Jmbulingam3, Panneerselvam Annamalai3, and Kalaichelvan PT1

1Centre for Advanced studies in Botany, Guindy Campus, University of Madras, Chennai 600025,Tamil Nadu, India

2Department of Botany, Periyar University, Periyar Palkalai Nagar, Salem – 636011, Tamil Nadu, India

3Department of Botany and MicrobiologyA.V.V.M Sri Pushpam College (Autonomous),Poondi - 613 503. Thanjavur (Dt.), Tamil Nadu, India

*Corresponding Author:
Murugesan Subban
Department of Botany, Periyar University, Periyar Palkalai Nagar, Salem – 636011, Tamil Nadu, India
Tel: +91 9486131494

Received date: 03/12/2013; Accepted date: 30/12/2013

 

Abstract

Bacterium exhibiting extra-cellular catalase activity was isolated from lime stone soil. Catalase is an essential component of the cell to cope up with oxidative stress. This study deals with simple detection method by plate assay for catalase activity from bacterium Acinetobacter calcoaceticus AV-6. Rapid assay method involves plating the supernatant on the substrate bound agar with the dye. This assay gave result within 60 s, producing a zone around the well. This enzyme was extracted from stationary phase at 36 h. The molecular weight of the protein was determined to be approximately 60 kDa. Sequencing studies were carried out and the strain Acinetobacter calcoaceticus AV6 was partially sequenced. The results imply the significance of this detection method in industries.

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