Analysis of the Cytochrome b Gene in Some Obese and Non Obese Saudi Arabian Populations
- Corresponding Author:
- Sayed AM Amer
Faculty of Science
Department of Biology
Taif University, Saudi Arabia
E-mail: [email protected] yahoo.com
Received Date: November 06, 2015; Accepted Date: January 06, 2016; Published Date: January 12, 2016
Citation: Amer SAM, AlHothali BRA, Tubaigy SMA (2016) Analysis of the Cytochrome b Gene in Some Obese and Non Obese Saudi Arabian Populations. J Forensic Res 6:318. doi: 10.4172/2157-7145.1000318
Copyright: © 2016 Amer SAM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Forty six Saudi samples were collected from obese and non-obese males aged between 25 and 35 years old. Approximately 1000 bp of the mitochondrial cytochrome b gene for these samples were amplified and sequenced as an initial effort to determine the sequence variation within this mtDNA coding region. The sequenced fragment was aligned with its counterparts from 100 healthy African and Asian humans found in the Genbank database. The polymorphisms of these sequences were analyzed for forensic purposes. A total of 12 polymorphic sites and 25 variations were noted. Sixteen out of the 25 variations were synonymous and the other 9 encoded different amino acids (non-synonymous). The haplogroup of all samples was determined using Mitomaster software. A total of 5 common different haplogroups were observed (H2a, U5a, JT, L3 and R0a). The recorded SNPs were tribe other than obesity-dependent except one common SNP (C15452A) which seemed to be an obesity marker, however, it was also found in the non obese individuals. Although the polymorphisms of cytochrome b gene were observed in Saudi populations and could be considered as a powerful forensic marker, however, more samples must be analyzed to investigate the unique distribution for forensic applications.