alexa Anti-Diabetic Action of Cydonia oblonga Seed Extract: I
e-ISSN: 2321-6182 p-ISSN: 2347-2332

Research & Reviews: Journal of Pharmacognosy and Phytochemistry
Open Access

Like us on: https://twitter.com/j_phytoch
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Anti-Diabetic Action of Cydonia oblonga Seed Extract: Improvement of Glucose Metabolism via Activation of PI3K/AKT Signaling Pathway

Dan Tang1, Lian-Zhen Xie1,2, Xue-Lei Xin1, and H. A. Aisa1*

1The Key Laboratory of Plant Resources and Chemistry of Arid Zone and State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, People’s Republic of China

2University of Chinese Academy of Sciences, Beijing 100039, People’s Republic of China

*Corresponding Author:
H. A. Aisa
Xinjiang Technical Institute of Physics and Chemistry
Chinese Academy of Sciences
40-1 Beijing Road, Urumqi
Xinjiang 830011
P. R.,China
E-mail: [email protected]

Received date: 12/04/2016; Accepted date: 10/06/2016; Published date: 17/06/2016

 

Abstract

Context: Diabetes mellitus is one of the most common metabolic syndromes characterized by hyperglycemia. Cydonia oblonga Miller (Rosaceae) is used in traditional uyghur medicine and is proved to have antioxidant and antihypertensive properties, but the anti-diabetic activity of C. oblonga Mill. seeds extracts (CSE) is not yet explored.

Objective: The anti-diabetic ability of CSE and the underlying mechanism of action of CSE were investigated in L6 skeletal muscle cells.

Methods: CSE were evaluated for their inhibitory activity against protein tyrosine phosphatase 1B (PTP1B) in vitro. L6 myoblasts were differentiated to myotubes and treated with CSE (0 μg/ml to 100 μg/ ml) for 1 h. Cell viability was assessed by MTT assay and cell cytotoxicity was detected by lactate dehydrogenase (LDH) release from L6 cells. The phosphorylation levels of four key proteins (IRS-1, AKT, GSK-3β and AMPK) involve in PI3K/AKT signaling pathway were examined by western blotting.

Results: CSE possess good anti-PTP1B activity with an IC50 value of 3.5 μg/mL. Treatment of L6 skeletal muscle cells with CSE (0 μg/ml to 100 μg/ml) did not affect cell viability. CSE at 12.5 μg/ml increased glucose consumption and glycogen synthesis in L6 myotubes. Western blotting results showed that CSE increased the phosphorylation of IRS- 1, AKT and GSK-3β protein in the present of insulin.

Discussion and Conclusion: These results suggested that CSE promoted glucose metabolism by activating PI3K/AKT signaling pathway in L6 cells. The study reported the anti-diabetic effect of CSE on in vitro cell model for the first time, which providing potential drug candidates for prevention and treatment of diabetes.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords