Assessment of Genotoxicity and Histopathological Changes Induced by Polyethylene Glycol (PEG6000) in Male Mice
- *Corresponding Author:
- Aida I ElMakawy
Cell Biology Department
National Research Centre, Giza, Egypt
E-mail: [email protected]
Received Date: June 21, 2012; Accepted Date: August 25, 2012; Published Date: August 27, 2012
Citation: Diab KAE, Elmakawy AI, Abd-Elmoneim OM, Sharaf HA (2012) Assessment of Genotoxicity and Histopathological Changes Induced by Polyethylene Glycol (PEG6000) in Male Mice. J Cytol Histol 3:153. doi:10.4172/2157-7099.1000153
Copyright: © 2012 Diab KAE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Polyethylene Glycols (PEG) the synthetic polymers are intended for use as plasticizers in aqueous film coatings used in the preparation and formulation of food supplement products, in coating of pharmaceutical products, as carrier for sweeteners, in various types of approved medical products, such as laxatives and ophthalmic products.
Authorization of polyethylene glycols for use in pharmaceutical products and food supplements focuses the attention
to verify the safety of this compound. So the aim of this study is to estimate the genotoxicity and histopathological
effects of polyethylene glycol in male mice. The study was conducted by comet assay and micronucleus test in bone
marrow cells, diphenylamine assay of DNA fragmentation in liver tissue and histological, histochemical examination
in liver and kidney cells. Results showed that PEG6000 exhibited DNA damage, represented in dose-dependent
increase in %DNA in comet tail, tail moment and olive tail moment, micronuclei number, DNA fragmentation
and decrease in DNA content of liver and kidney cells. Histopathological examination showed dose dependent
degenerative changes in both of liver and kidney cells. The histopathological changes were represented in vacuolar degeneration, few inflammatory cellular infiltration and necrosis and pyknotic nuclei in liver cells and sever tubular
degeneration and vacuolar degeneration in their tubular epithelial cells and cloudy degeneration, marked interstitial
cellular infiltration in kidney cells. Histochemical study showed that PEG600 caused significant decrease in DNA
content in liver and kidney cells. We concluded that the repeated exposure to high dose of PEG6000 shows genotoxic
activity and histopathological changes in male mice cells. So that special attention must be paid to legalization of
indiscriminate use of this compound to reduce the risk affecting human health.