alexa Bioproduction, Antimicrobial and Antioxidant Activities
ISSN: 2320-0189

Research & Reviews: Journal of Botanical Sciences
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Research Article

Bioproduction, Antimicrobial and Antioxidant Activities of Compounds from Chlorella vulgaris

Danielli M.M. Dantas1, Romero M.P.B. Costa1,2, Maria G. Carneiro-da-Cunha1,2, A. O. Galvez3, A. R. Drummond4, and Ranilson S. Bezerra1*

1Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Pernambuco (UFPE), PE, Brazil

2Laboratorio de Imunopatologia Keizo Asami–LIKA/UFPE, CEP 50.670-901 Recife, PE, Brazil

3Departamento de Aquicultura e Pesca, Universidade Federal Rural de Pernambuco (UFRPE), PE, Brazil. 4Laboratorio de Fluidos, ITEP, PE, Brazil

*Corresponding Author:
Ranilson S. Bezerra
Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Pernambuco (UFPE), PE, Brazil
Tel: +55.81.2126.8540
E-mail: [email protected]

Received date: 13 August 2015 Accepted date: 14 September 2015 Published date: 16 September 2015

 

Abstract

Various crude extract preparations (ethanol, methanol, buthanol, acetone, DMSO and water) from the green alga Chlorella vulgaris were examined for Antioxidant activity, Phytochemical screening and Antimicrobial properties. In vitro free radical quenching and total antioxidant activity of extracts were investigated with 1, 1-diphenyl-2- picryl hydrazyl (DPPH), and compared with cathequin and Gallic acid as positive controls. In most cases, results showed a significant association between the antioxidant potency and the total phenolics content. The aqueous extract showed both the highest antioxidant activity for inhibition scavenging (68.5%) and highest phenolic content (3.45 mg/ mL). Antimicrobial activities were carried out using disc diffusion assays and the broth dilution method against Gram-positive and Gram-negative bacteria. Results demonstrated activity between the aqueous extract and most specimens (Proteus mirabilis, Klebsiella pneumoniae, Salmonella enteretidis, Bacillus subtilis and Escherichia coli). These results suggest that the aqueous crude extract of C. vulgaris could be considered as a biological antioxidant and antimicrobial agent, and a valuable tool for the biotechnology field.

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