alexa Complexity of Odontoblast and Subodontoblast Cell Layers in Rat Incisor
e-ISSN: 2320-7949 and p-ISSN: 2322-0090

Research & Reviews: Journal of Dental Sciences
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Complexity of Odontoblast and Subodontoblast Cell Layers in Rat Incisor

Alhelal A1*, Mahdee A1, Eastham J2, Whitworth J3, Gillespie JI4

1PhD student, Centre for Oral Health Research, Institute of Cellular Medicine, School of Dental Sciences, UK

2Research assistant, School of Dental Sciences, UK

3Clinical Consultant/Senior Lecturer, Centre for Oral Health Research, School of Dental Sciences, UK

4Professor of Human Physiology, School of Dental Sciences, UK

Corresponding Author:
Alhelal A
School of Dental Sciences, Faculty of Medicine, Newcastle University, Newcastle upon Tyne NE2 4BW, UK.
E-mail: [email protected]

Received date: 17/12/2015 Accepted date: 19/02/2016 Published date: 26/02/2016



Structural and functional interrelationships between odontoblast (OCL) and sub-odontoblast cell layers (SOCL) are relevant to tissue formation and regulation in health and disease, but are incompletelyunderstood. This study employed contemporary immunohistochemical techniques to gain important new insights on tissue complexity within the apical half of the rodent mandibular incisor. Whole incisors were removed from freshly killedWistar rats, fixed in 4% paraformaldehyde,demineralized, and prepared for standard immunohistochemistry. Tissues were labelled with primary antibodies to vimentin, α actin, NaKATPase, alpha tubulin and COX 1, and secondary antibodies alexafluor 488 and 594, before examination by fluorescence microscopy. Odontoblasts presented in stratified or pseudo-stratified arrangements, rather than simple columnar form. Additional stellate cells,α-actin immunoreactive, were observed predominantly in the distal half of the OCL. Cells in the cell rich zone (crz) also showed complexity, with high cell density, and cells presenting with large nuclei and sparse cytoplasm. These cells showed intense NaKATPase immunoreactivity. Some of these cells sent processes towards and into the OCL, whilst other processes remained within the crz. No processes directed downwards. The OCL of the rat mandibular incisor presents a hitherto undescribed complexity, in terms of cellular arrangement and composition, the functional significance of which is not immediately apparent. The observation that cellular processes from crz tothe OCL and lateral processes within crz suggest functional connectivityand cell to cell communication within the layer. The intense immunoreactivity to NaKATPase suggests functional specification and a high degree of tissue activity. Structural observations will require further investigations.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version