Cytotoxic activity of crude and partially purified ink of L.duvauceli towards HepG2cell line
The present study aims to evaluate the anticancer activity of the crude and partially purified protein fractionated ink of Loligo duvauceli on the Hep G2 cell line using cell viability and cell proliferation assay (MTT assay). Different concentrations of the ink were prepared (125μg, 250μg, 500μg) and the cytotoxic activity was determined. Among the three doses tested, the crude ink of L.duvauceli at the concentration of 500 μg/ml was found to inhibit the viability of the cell effectively and the percentage of viability ranged between 33 – 41. Cells viability decreased with increasing concentrations of the samples. HepG2 cells treated with protein fractionated ink, decreased the percentage of viability and the percentage ranged from 30-49. MTT assay revealed that partially purified ink of L. duvauceli showed higher activity than that of crude ink. The percentage of cell death was 70 in partially purified protein fractionated ink and 67% in crude ink at a concentration of 500μg. IC 50 value was obtained at a concentration of 125μg. The present findings suggest the profound anticarcinogenic activity of partially purified ink of L.duvauceli on HepG2 cancer cell line. Further purified compounds can be used as a potential chemotherapeutic drug for the treatment of Hepatic cancer.