Effect of Seed Direction and Growth Media on In Vitro Seeds Germination and Seedling Establishment of Pterocarpus Marsupium Roxb.
In present study in vitro seed germination methods were developed for optimization of germination performance of medicinally important plant, Pterocarpus marsupium Roxb. In vivo seed germination through conventional method of this plant and seeds are facing so many problems due to its hard seed coat and poor viability. Application of PGRs is also not much affective in in vivo condition. Present study was conducted using in vitro culture technique the seeds were inoculated in different orientation in to different media type viz. M.S media, ½ M.S, Nitsch media. Among above results media and horizontal direction was found suitable for seed germination. So, with that selected condition different hormones were added in media for better germination. All together horizontal direction on MS media are the good for the population of the plant.