alexa Enzyme-assisted Extraction of Polyphenols from Pomegran
E- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286

Research & Reviews: Journal of Microbiology and Biotechnology
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Research Article

Enzyme-assisted Extraction of Polyphenols from Pomegranate (Punica granatum) Peel

Mushtaq M1, Sultana B1, Akram S1, Adnan A1, Owusu-Apenten R2 and Nigam Singh P2*

1Department of Chemistry, University of Agriculture, Faisalabad-38040, Pakistan

2School of Biomedical Sciences, Ulster University, Coleraine, BT52 1SA, UK

*Corresponding Author:
Nigam Singh P
School of Biomedical Sciences
Ulster University, Coleraine
BT52 1SA, UK
E-mail: [email protected]

Received date: 02/05/2016; Accepted date: 26/05/2016; Published date: 06/06/2016



Objective: This study examines enzyme-assisted recovery of polyphenols from pomegranate peel using a multi-response optimization process. Methods: Enzyme pre-treatment factors (enzyme dose, incubation time, temperature and pH) were optimized for extract yield, total phenol concentration (TPC), radical scavenging capacity (RSC) and trolox equivalent antioxidant capacity (TEAC). Results: Pre-treatment of pomegranate peel with 3.8% of cocktail enzyme at 6.7 pH and 41ºC for 85 min produced a mass yield of 65.89 ± 2.64 g/100 g of crude extracts (3 fold increase compared to conventional solvent extraction) with a total phenols concentration of 277.93 ± 6.17 mg GAE/g dry weight, 398.70 ± 3.06 μmol trolox equivalence /g and 73.15 ± 0.69 μg/mL radical quenching capacity (IC50). Conclusion: Enzyme pre-treatment improved the efficiency of extraction of polyphenols from pomegranate peel.


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