Evaluation of Antianxiety Activity of Xanthine Oxidase Inhibitors in Albino Mice
Background: Anxiety the most common mental health disorder affecting majority of the population. Serotonin is involved to be a major neurotransmitter in causing anxiety. Tryptophan is the only precursor of serotonin and xanthine oxidase is the endogenous activator of tryptophan pyrrolase enzyme which can result in reduced serotonin level. Xanthine oxidase inhibitors putatively inhibit the metabolism of tryptophan therefore leading to increase in serotonin level. Hence the study was planned to evaluate the antianxiety effect of xanthine oxidase inhibitors, allopurinol and febuxostat. Objective: To evaluate the antianxiety activity of Allopurinol 39mg/kg and Febuxostat 15.6mg/kg in comparison with control, Diazepam0.5mg/kg and with Fluoxetine10mg/kg. Methods: Elevated plus maze-Pre-treated animals were placed individually for 5mins in the maze. The number of entries into the open and closed arm, time spent in each arm and the no. of entries in both the arms was noted. Social interaction test-Pre-treated mice were isolated for 1hour before the test. In the test arena, the mice were observed for cumulative time spent in social interaction for a period of 10mins. All the results are expressed as Mean±SEM. Data are analyzed by ANOVA using Graph Pad Instat (GPIS) package, version 3.05. P < 0.05 was considered as significant. Results: Elevated plus maze- Administration of Allopurinol and Febuxostat significantly increased the time spent in open arms, p<0.001&p<0.018 respectively in comparison with control. Social interaction test- Administration of Allopurinol and Febuxostat significantly increased the time spent in social interaction, p<0.001 as compared to control. Conclusion: Both Allopurinol and Febuxostat possessed significant antiaxiety effects.