alexa In Vitro Callus Induction and Antimicrobial Activities
ISSN: 2322-0066

Research & Reviews: Research Journal of Biology
Open Access

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Research Article

In Vitro Callus Induction and Antimicrobial Activities of Callus and Seeds Extracts of Nigella Sativa L

Mawahib E.M. ElNour1*, Futooh Z. A. Mahmood1and Sanaa O. Yagoub2

1Department of Biology and Biotechnology, Faculty of Science and Technology, AL Neelain University, Khartoum, Sudan

2Department of Microbiology and Molecular Biology , Faculty of Science and Technology, AL Neelain University, Khartoum, Sudan

Corresponding Author:
Mawahib El Amin Mohamed ElNour
Department of Biology and Biotechnology, Faculty of Science and Technology, AL Neelain University, Khartoum, Sudan.
Mobile:
00249923986335
E-mail: [email protected]

Received date: 21/05/2015 Accepted date: 19/08/2015 Published date: 24/08/2015

 

Abstract

In this study, extracts of Nigella sativa seeds and its induced callus were investigated for their antimicrobial activities against four standard bacteria (Bacillus subtilus, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) and two fungi (Candida albicans and Aspergillus niger) by using agar diffusion method. To induce callus, hypocotyls and cotyledons explants from N. sativa were cultured in MS medium supplemented with different types and different concentrations of growth regulators. Explants of N. sativa showed a rapid rate of initiation of callus after two weeks when grown in MS media supplemented with NAA at 1.0 mg/l and 5.0 mg/l of NAA respectively, while a slow rate of induction of callus observed when the hypocotyls grown in MS media supplemented with 5.0 mg/l 2, 4-D and 0.5 mg/l 2, 4-D, when the explants were cotyledons. The NAA in this study was found to be the suitable hormone regulator for N. sativa for both types of explants used. Methanolic extracts of seeds and callus of N. sativa showed activity against Escherichia coli with inhibition zone (21 mm) and (23 mm) respectively and no antifungal activity was observed for both seeds and callus extracts. The antibacterial activity of Penicillin and Gentamicin were determined against the tested bacteria and compared with the antibacterial activity of the tested extracts of N. sativa seeds and callus. Methanolic extracts show antimicrobial activity against E. coli higher than that of Gentamicin and Penicillin at 10 µg/disc. Phytochemical screening for the seeds and callus extracts indicated the presence of secondary metabolites such as alkaloids, flavonoids and tannins which may be responsible for the antimicrobial activity of the tested extracts.

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