alexa Isolation and Antimicrobial Evaluation of Bixa orellana
E- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286

Research & Reviews: Journal of Microbiology and Biotechnology
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Research Article

Isolation and Antimicrobial Evaluation of Bixa orellana and Linum usitatissimum

Mital Patani1*, Mandev Patel2, Khomendra Sarwa3, and Anil Bhadari1

1Jodhpur National University, Jodhpur, Rajasthan, India

2A-One pharmacy College, Naroda. Gujarat, India

3Dibrugarh University, Dibrugarh-786004, Assam, India

*Corresponding Author:
Mital Patani
Jodhpur National University, Jodhpur, Rajasthan, India.
Mobile: +91 9099036562

Received date: 01/04/2013 Accepted date: 25/04/2013



This research work was carried out to investigate the antimicrobial activities of isolated fraction of Bixa orellana and Linum usitatissimum against five bacterial and five fungal pathogen. Leaves Powder of bixa orellana and seed powder of Linum usitatissimum subjected to soxhlet extraction. The separation of various fraction was carried out by column chromatography, analyzed and purified by thin layer chromatography(TLC) until homogeneity of the fraction achieved. The homogeneity and the purity of the different fractions were checked by HPLC. Antimicrobial evaluation of isolated fractions was carried out. Two fraction Fr-1 (352mg) and Fr-2(260mg) were collected from Bixa orellana had Rf was o.99 and 0.35. fraction 3 and 4 was isolated from linum usitatissimum with Rf 0.45 and 0.25. When compare to the standard drug gentamycin, phenolic fraction of 1 and 2 had good antibacterial activity against all bacterial strain where as glycoside fraction 3 and 4 were poorest activity against all bacterial strain. When compare to standard drug tioconazole fraction-1 and 2 had very good activity against fungal strain and fraction 3 and 4 had moderate activity against fungal strain.


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