alexa Molecular Cloning and Characterization of Two Key Enzym
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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Research Article

Molecular Cloning and Characterization of Two Key Enzymes involved in the Diterpenoid Biosynthesis Pathway of Isodon rubescens

Xiuhong Su1,2, Lei Yin1 and Suiqing Chen1,2*

1Pharmacognosy Discipline, College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, Henan, China

2Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment and Chinese Medicine Development of Henan Province, China

*Corresponding Author:
Suiqing Chen
Pharmacognosy Discipline, College of Pharmacy
Henan University of Traditional Chinese Medicine
Zhengzhou 450046, Henan, China
Tel: +8637165676686
E-mail: [email protected]

Received Date: June 16, 2017; Accepted Date: June 22, 2017; Published Date: June 26, 2017

Citation: Su X, Yin L, Chen S (2017) Molecular Cloning and Characterization of Two Key Enzymes involved in the Diterpenoid Biosynthesis Pathway of Isodon rubescens. J Anal Bioanal Tech 8:369. doi: 10.4172/2155-9872.1000369

Copyright: © 2017 Su X, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Isodon rubescens, an important medical plant, contains various terpenoids. This plant’s active compounds are primarily oridonin with antitumor properties. As the precursor for oridonin biosynthesis, are synthesized by MEP pathway. On the basis of our earlier studies, we isolated and cloned two important genes catalyzing diterpenoid biosynthesis in the MEP pathway. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase and 4-hydroxy-3- methylbut-2-enyl diphosphate reductase are the fifth enzymes and the last step key enzyme for the methylerythritol phosphate (MEP) pathway, respectively, which is important for the regulation of isoprenoid biosynthesis. Sequence analysis revealed that DcIspF (accession no. KT948057) was 966 bp, contains a gene open reading frame (ORF) of 708 bp belonging to the MECDP-synthase superfamily and DcIspH (accession no KT948058) contains a 1389 bp ORF encoding a predicted 462 amino acid polypeptides as a member of the lytB_ispH superfamily. The deduced DcIspF and DcIspH amino acid sequences shared high similarity with DcIspF and DcIspH of other plant respectively, each of them exhibiting an N-terminal transit peptide and conserved amino acid sites. Quantitative real-time PCR analysis showed that the expression of DcIspF was considerably higher in leaves, the lowest in callus. These results indicate that we have identified functional DcIspF and DcIspH enzymes, which may play a pivotal role in the biosynthesis of diterpenoid in I. rubescens.

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