Pharmacological Study of a Steroid-sitosterylglucoside-6-heptadecanoate Isolated from Cappris horrid
Pharmacological properties of the species Capparishorrida has been known among the tribal population of Odisha since long.The aqueous extract of this plant material is related to anti inflammatory and analgesic activities. It has also been known for its medicinal properties as anthelmintic diuretic and aparentic. The ethanolic extract of this plant species was subjected to chromatographic techniques using silica gel and benzene: petroleum benzene (1:9) to isolate a new steroid -sitosterylglucoside-6′-heptadecanoate as the major compound. The TLC was studied using ethyl acetate : benzene (1:9) the Rf value of the compound is 0.63. The structure of the compound was established from the IR, H-NMR, 13C-NMR and mass spectral data. The elemental analysis of the compound supported these spectral data. The bioassay of the isolated material was studied using frog rectus abdomonis muscle. The acetycholine liberated from the nerve ending of the muscle caused a depolarization of the muscle fiber. From the Kymograph it is observed that the compound caused muscle contraction in frogs. The antimicrobial activity was studied by two fold series dilution method. The anti bacterial activity of the compound was compared with Gentamycin which is a known drug having this activity. The compound showed bacterial growth inhibiting property.