Purification of coagulant proteins from Moringa Oleifera using Nanostructured Zirconium as a bioadsorbent
G Vijaya kumar1, V Krishna2, Sukumar Roy3 and Pushpa Agrawal4
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The coagulant protein from Moringa oleifera seeds were purified using nano-structured zirconium (NSZ) particles as a bio-adsorbent by conducting continuous bed adsorption studies based on the Isoelectric Point (pI) of the zirconium and coagulant protein. The characterization of purified protein was done by conducting COD of waste water and results were compared with proteins purified by standard IEX chromatography method and the characterization of NSZ particles was done by Scanning Electron Microscope. The adsorption isotherms of purified protein on NSZ particles at different adsorbent concentration at pH 7.0 were experimentally obtained by batch studies the results follows the Freundlich and Langmiur isotherm adsorption model. The series of column tests were performed to determine the breakthrough curves at different bed depths. The results indicated that the NSZ particles were effective in purifying the proteins based on pI.