Quantitation of chlorogenic acid from aqueous and methanolic extracts of wood apple (Aegle marmelos C. Linn) by GC-MS Ion Â– trap
C.Rajeshkannan1 and G.Lakshmanan2
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GCMS is a robust and very widely used technique that combines high sensitivity and specificity for suitable chemical constituents. The identification and quantitation of chemical constituents has been done by GC/MS Ion-trap with respect to the internal and external standard. The internal standards are 60000 patterns of reference compounds and its reference spectrum of NIST library. The present study has been conducted for the quantification of chlorogenic acid (CGA) from the aqueous and methanolic extracts of wood apple (A.marmelos) with external standard by GC-MS Ion trap. Chlorogenic acid is a targeted compound, water soluble phenolic compound it plays vital role in physiological functions in plants and pharmacological activities in humans. The total mass of chlorogenic acid is 354. It would be identified and quantified from different extract of wood apple in comparison with external standard. Retention time of standard chlorogenic acid, aqueous extract, methanolic extract and combined extracts indicates 43.237, 43.236, 43.234 and 43.241 minutes respectively. Mass spectral data of standard fragmented ions are 311.3, 312.3, 325.3, 354.2, 355.0 and these ions were found in all the samples of wood apple. Therefore, quantitation of target compound with respect to the external standard, its similarity of retention time and mass spectral data between the standard and samples would be used for the identification and quantification of target compound. The targeted compound has been quantified as 41.9, 9.5 and 29.7 percent of aqueous extract of WA, methanolic extract of WA and combined extract of WA respectively. GCMS is a selective and sensitive analytical method to identify and quantify the target compound in chemical matrix with reference to internal and external standard of chemical constituents. Target component possess wide application of medicinal properties, especially in psychiatric disorders and this analysis might be used to know the exact concentration of target chemical components in the aqueous and methanolic fruit extract of A.marmelos (wood apple).