Screening of Glycolytic Enzyme Inhibitory Activity of Streptomyces Isolates from Brine Spring and Marine Sediments of India
The objective of the study was to investigate the percentage inhibition of glycolytic enzymes by compounds produced by two strains of Streptomyces sp. isolated from different niches of India. The strain Streptomyces sp. VITPK9 was isolated from brine spring located in Thoubal district, Manipur, India and the strain Streptomyces sp.VITSTK7 was isolated from marine sediments collected from Puducherry coast, Tamil Nadu, India. Both the strains were characterized using molecular taxonomy and identified as Streptomyces sp.VITPK9 (JN689333) and Streptomyces sp.VITSTK7 (GQ499369). The 16S rDNA analysis showed 97% similarity of the strain VITPK9 with Streptomyces pseudogriseolus (X80827) and 86% similarity of the strain VITSTK7 with Streptomyces longisporoflavus (DQ442520). The strains were fermented for seven days using Kuster’s broth with pH 7-7.2 at room temperature in shake flask incubator at 150 rpm. The ethyl acetate (EA) extract obtained from isolates were screened for α-amylase and α-glucosidase inhibitory activity. The EA extract (500 μg/ml) of VITPK9 showed significant (69.1%) inhibitory activity against α-amylase and compared to VITSTK7 activity. The EA extract (500 μg/ml) of VITSTK7 inhibited (64.3%) α-glucosidase and compared to VITPK9. From this study we are concluding that both the extracts showed concentration dependent inhibitory activity against tested glycolytic enzymes. The bioactive compounds need to be extracted and identified from these potential isolates to study its mechanism of action.